Development and Verification of an Integrative Vector for the Industrial Osmotolerant Yeast Candida glycerinogenes

Abstract:

To express heterogeneous genes under the regulation of osmotic pressure in Candida glycerinogenes CGMCC NO. 6830，an integrative vector was constructed in this study. The constructed integrative vector harbored the backbone of plasmid pUC19，18S rDNA sequence of C. glycerinogenes CGMCC NO. 6830 as the integration site，phleomycin-resistant gene（ble） as a selectable marker，the osmolarity regulated promoter（PCggpd） and green fluorescent protein gene gfp as a reporter. The target gene was integrated into chromosomes of C. glycerinogenes CGMCC NO. 6830 and the expression level of gfp was regulated by osmotic pressure.