Screening of PVA-Degrading Strains and Optimization of Degradation Conditions

Abstract:

In order to achieve the biodegradation of PVA1799 in the textile industrial waste water，an bacteria mixture with efficient degradation was screened and named as SH-TD. Three strains with PVA-degrading ability were isolated from the mixed culture SH-TD and nasmed as SH1（Aeromonas sp.），TD5（Pseudomonas sp.），and TD33（Acinetobacter sp.），respectively. The results from the single factor and Plackett-Burman experiments showed that Yeast-Extract，（NH4）2HPO4 and BaCl2 constituents have the significant influence on PVA degrading rate. Through ascent and Box-Behnken experiments，a response surface methodology（RSM） model was constructed. Data were analyzed by use of Design-Expert software. The optimal concentrations（g/L）of the variables were determined as （g/L）：Yeast-Extract 0.04，（NH4）2HPO4 48.42，BaCl2 0.06. Under the optimal conditions，the PVA degradation rate after 24 h increased from 30% to 80% .