Effects of 9-Cis-Retinoic Acid on the Proliferation and Apoptosis of Human Lung Cancer Cell Line A2

Abstract:

The study aimed to explore the mechanism of 9-cis-retinoic acid(9-cis RA) inhibiting proliferation and inducing apoptosis in human lung cancer cell line A2.Cultured A2 cells were randomly divided into four groups，the experimental group received 9-cis RA to a final concentration of 1，5 or 10 μmol/L，and the control group was added dimethyl sulfoxide to a final concentration of 0.1%. The MTT method was used for the determination of cell proliferation，flow cytometry(FCM) for the analysis of apoptosis，and reverse transcription-polymerase chain reaction(RT-PCR) for the expression changes of bax，fas and bcl-2 genes. Results showed that 9-cis RA significantly inhibited the cell proliferation(P<0.01) and the incidences of apoptosis in the experimental groups were significantly higher(P<0.05)，which depended on the concentration of 9-cis RA.The mRNA expressions of bax and fas genes in the experimental groups were significantly higher(P<0.01) whereas the bcl-2mRNA expressionwas significantly lower than the control group(P<0.01).Therefore，9-cis RA significantly inhibitsthe proliferation of human lung cancer cell line A2，which may be related to the increased expressions of bax and fas genes and the decreased expression of bcl-2 gene，and then induced apoptosis.