Determination of 14 Pesticide Residues in Fresh Jujube Using Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry

Abstract:

This study developed a method to identify 14 pesticides residues(including organic phosphorus insecticides，fungicides，organic heterocyclic pesticides and novel nicotine insecticide) in fresh jujube by solid-phase extraction and liquid chromatography coupled with electrospray ionization tandem(HPLC-MS/MS)，in which a strong hydrophobic silica bonded tC18 column was selected as the solid-phase extraction，electrospray ionization(ESI) was used as the ion source and positive ion multiple reaction monitoring(MRM) was set as the detection mode. The effects of different extraction solvents and solid-phase extraction columns were compared for the determination of 14 pesticides residues，and at the meanwhile the separation and detection conditions for the multiresidue analysis by chromatography and mass spectrometry were also optimized. A good linear relationship could be achieved for the detection of 14 pesticides in fresh jujube in the range of 10~500 μg/L (or 1~50 μg/L)，with the correlation coefficients of 0.996 3~0.999 9，the quantitation limits of 0.10~9.50 μg/kg，the recoveries of 67.4%~116.8% for 14 pesticides of three different concentrations，and RSD of 0.8%~14.8%. The optimized method，which is applicable to determine 14 pesticide residues in fresh jujube，meets the requirements of the multiresidue analysis with simple operation，good sensitivity，and high accuracy.