Maillard Reaction Between Glucose and Histamine and Its Inhibition on Cell Proliferation and Effect on Cell Cycle of Human Hepatic Cell Line (HL7702)

Abstract:

The effects of Maillard reaction products(MRPs) and histamine on the inhibition of cell proliferation and cell cycle of human HL7702 were investigated. Human HL 7702 cells were respectively treated by MRPs and histamine with different concentrations for 24，48 and 72 h，i.e.，0.2 mg/mL、0.4 mg/mL、0.6 mg/mL、0.8 mg/mL、1 mg/mL for MRPs，and 0.047 mg/mL、0.094 mg/mL、0.141 mg/mL、0.188 mg/mL、0.235 mg/mL for histamine. The inhibition of cell proliferation induced by MRPs and histamine was detected by MTT assay. The effects of MRPs and histamine on the cell cycle distribution in human HL7702 were detected with flow cytometry using Annexin assay. The inhibition of cell proliferation induced by MRPs and histamine was dependent on the treatment concentration and duration，in which the inhibition was observed after 24 h、48 h、72 h treatment in human HL7702. Histamine was a stronger inhibitor compared with MRPs. The cell cycle arrest was affected by both MRPs and histamine，while histamine was more effective. In conclusion，MRPs could significantly reduce the histamine-induced cytotoxicity of human HL-7702 cells.