Optimization and Industrial Production of Catalase in Bacillus subtilis

Abstract:

Microorganism catalase,which is an important industrial enzyme,plays an important role in textile industry. To realize the industrial production of catalase,the fermentation on B. subtilis WSHDZ-01(pSTOP1622-katA),which was constructed before,was conducted in 3 L fermentor. Furthermore,the enzyme activity reached 35 398 U/mL after optimization of carbon and nitrogen concentration,which was increased by 53.41% compared with that before optimization. Based on this result,the fermentations in 24 L and 500 L fermentor were carried out,respectively and the maximum enzyme activity was 28 940 U/mL and 23 190 U/mL respectively. The result showed the yield of catalase has met the requirement of industrial production,which lay a solid foundation for larger-scale industrial production.