Fermentation Medium Optimization for Arginine Deiminase Production from Recombinant Escherichia coli by Response Surface Methodology

Abstract:

Response surface methodology was used to optimize the fermentation medium of recombinant E. coli for arginine deiminase production on the basis of single factor optimization. Five medium factors were optimized by SAS software combined with Plackett-Burman and Box-Behnken design. The optimized fermentation medium contains 11.16 g/L tryptone,20 g/L yeast extract,6.3 g/L glycerol,16.38 g/L K2HPO4,and 2.31 g/L KH2PO4. Using the optimized medium,the activity of ADI reached 5.7 U/mL broth,which was 1.53-fold higher than that of in the initial medium (3.72 U/mL)and 2.01-fold of that in LB medium(2.83 U/mL). In a 3-L bioreactor,ADI activity of 15.17 U/mL broth was attained using optimized medium,which was remarkably enhanced compared with that of LB medium (4.32 U/mL).