In vitro Metabolism of Soyasaponin II by Human Intestinal Microflora

Abstract:

To investigate the metabolism of soyasaponin II by human intestinal microorganisms,the in vitro metabolic transformation of soyasaponin II by intestinal flora in the Brain Heart Infusion(BHI)broth and Phosphate Buffered Saline(PBS)systems was studied for 48 hours,and its metabolites were analyzed and identified by HPLC/MS. The results showed that soyasaponin II was firstly metabolized into soyasaponin IV by intestinal flora and was eventually translated into soyasapogenol B at 48 hours. The above results suggested that soyasaponins lost their sugar moieties due to hydrolysis and yielded smaller and more hydrophobic metabolites by human gut microorganisms.