TAS-ELISA and Electrochemical Paper Assisted Immunosensor for the Detection of Watermelon Mosaic Virus

Abstract:

Using a mice monoclonal antibody and a rabbit polyclonal antibody of the watermelon mosaic virus (WMV)-2 coat protein, we developed two detection methods for WMV-2. The trimer antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) method was established based on two different antibodies; the electrochemical paper assisted immunosensor detection method was established by combining the electrochemical workstation with the paper assisted immunosensor prepared by the carbon nanotube and the antibody-coated filter paper. The detection limit was 0.15 and 0.2 μg/ml, and the testing time was 3 hours and 30 minutes for two methods, respectively. According to these results, the TAS-ELISA method had a higher detection sensitivity, and the electrochemical paper assisted immunosensor detection method had a shorter testing time.