Abstract:This manuscript was aimed to investigate the effect of γ-aminobutyric acid on bone performace in high-fat-diet-fed mice. For this,6-week-old C57BL/6 male mice(40) were randomly divided into four groups:normal diet group(C),high-fat diet group(HFD),mice in HFD supplemented with 0.06%,0.12% GABA by drinking water. A week prior to sacrifice,mice were placed in metabolism cages to collect urine for measuring urinary calcium and hydroxyproline content. Mice were sacrificed by 20th,femur,tibia were collected for measuring parameters,femoral oxidative stress and relative genes expression by RT-PCR. The results demonstrated compared with the control group,HFD showed femoral oxidative stress,down-regulated GAD65 and increased GABAbR1 expression as well as dramatically significantly up-regulated GSK3β and down-regulated Nrf2;In addition,mice in HFD showed significantly reduced femoral and tibial performance,while dramatically increased urinary calcium and hydroxyproline content. At the same time,osteoblast-specific genes Col1a1,BGLAP2 significantly down-regulated while osteoclast-specific genes expression RANKL/OPG,TRAP,CTSK increased significantly in HFD. 0.06%,0.12%GABA supplement can significantly recovered GSK3β,Nrf2,GABAbR1 expression,alleviated femoral oxidative stress,and thus improved femur and tibia performance accompanied by balanced bone metabolism. Conclusion:GABA can balance bone metabolism and partially recover bone performance by alleviating oxidative stress. 0.12% GABA showed a better effect than 0.06% in adjusting femoral redox state and improving bone performance.
