Near Infrared Fluorescent Probes and Their Applications in Immunoassay

Abstract:

Owing to the distinguished superiority of lower background noises,deeper penetrating capacity and less destructiveness to biomatrix over UV and visible fluorophores,near-infrared fluorescent probes(NIFPs) have gaining more and more attentions for analytical applications to date. With continuous research efforts in chemical synthesis and exploration of novel fluorescent materials,the number of NIFPs applicable for biological systems has grown substantially and widely applied in fields such as non-destructive detection,immunoassay and bioimaging in recent years. In this review,the properties,characterization and recent progresses in improvements of optical properties and signal intensity of 4 types of vital NIFPs(i.e. organic fluorophores,quantum dots,rare earth compounds and single-walled carbon nanotubes) were summarized. The applications of such important NIFPs in immunological analysis of environmental contaminants and clinically important biomarkers were also elaborated. Moreover,the potential of NIFPs-based immunochromatography technique adaptable for rapid detection of foodborne pathogens was also forecasted.