Screening and Medium Optimization of Bacterium Producing Neutral Cellulase

Abstract:

A neutral cellulase-producting bacterium was screened from soil,and this strain was identified as Bacillus through 16S rDNA sequence analysis. With it as a starting strain,we constructed a response surface methodology(RSM) model by single factor and factorial test,ascent experiment and central composite experiments. By this RSM model,we obtained the optimal medium containing 1.97% maltose,2.03% yeast extract-tryptone(1∶4,m/m) mixed nitrogen. The enzyme activity increased from 130.40 U/mL to 194.23 U/mL under the optimal conditions.