Isolation and Culture Optimization of Bacterial Cellulose Producing Strain Gluconacetobacter intermedius

Abstract:

Five bacterial cellulose(BC) producing strains were isolated from solid-state fermentation cultures of traditional Chinese vinegar,and identified as Gluconacetobacter intermedius based on their physiological and biochemical characteristics as well as 16S rDNA sequence analysis. Strain 1-17 could produce more BC then the others. Furthermore,surface features and chemical structure of BC were analyzed by scanning electron microscopy(SEM) and Fourier transform infrared(FT-IR) spectroscopy,respectively. Effects of temperature,time,carbon sources and initial pH on BC production were tested. The optimal temperature for strain 1-17 was 35 ℃,and the fermentation time was 7 d. Glucose and glycerol were the optimal carbon sources,and the optimal initial pH was 6.0. Both lactate and calcium could promote the synthesis of BC. The yield of BC was improved from (3.90±0.08) g/L to (7.90±0.19) g/L under the optimal conditions.