Study on Bacteriostasis and Anti-Tumor Effect of Isocorydine Base Derivate

Abstract:

To investigate the bacteriostasis activity and tumour inhibitory effect of semi-synthetic derivate of isocorydine 8-amino-isocorydine (8-NH2-ICD). The bacteriostasis activities of alkaloid in vitro were tested with agar diffusion method and minimal inhibitory concentrations (MIC). Inhibitory activity on proliferation of tumour cells with treated by 8-NH2-ICD were measured by MTT assay. The results show there is a dramatic bacteriostasis activity on Salmonella typhi and Escherichia coli, and relatively mild effect on Enterobacter aerogenes. MGC803 cell and M059K cell proliferation effect were dramatically inhibited by 8-NH2-ICD in vitro. The anti-tumour activity was gradually enhanced with the alkaloid variety of dose. However, 8-NH2-ICD have been demonstrated with low toxity to normal fibroblasts MRC-5 till concentration up to 200 mol/L. To our knowledge, this is the first time that 8-NH2-ICD has anticancer and antibacterial activity is reported.