Identification and Improvement of SOD Production for Bacillus cereus MBRH3

Abstract:

Three Microorganism with SOD production were separated from the seafloor mud of Jianmen harbour in Taizhou, Zhejiang Province, China. Thus, further studying on one of the stronger SOD activity strain was undertaken, Its phenotype, genetic and phylogenetic analysis experiments suggested that the strain belongs to the family Bacillaceae, genus Bacillus and named as Bacillus cereus MBRH3. A sequential statistical methodology comprising of Contral Composite Design were applied to enhance the fermentative production of SOD, As a result, maximum chitinase activity of 203.38 U/mL was obtained in the optimized medium concentration（g/L） of 4.5 corn steep powder, 4.11 Sea weed paste, 2.10 glucose and 0.62 magnesium sulfate. Bacteria was deposited into China Center for Type CultureCollection（CCTCC NO：M2013581）.