Abstract:To overcome the poor water solubility or lack of tumor-targeting of Doxorubicin（DOX）, we designed and fabricate the redox-sensitive K5 polysaccharide micelles as drug carrier, methods：An amphiphilic K5PS-SS-DOCA（KSD） conjugate was designed and prepared by covalently coupling deoxycholic acid（DOCA） with K5 polysaccharide（K5PS） via disulfide bonds. The conjugate could self-assemble into micelles in aqueous solution and encaspulate model drug DOX. The morphology of the micelles was observed by TEM, and the size and ζ-potential were measured by DLS. Results：The micelles were of spherical shape. The average size and ζ-potential was 225 nm and -31 mV for the micelles, and 241 nm and -31 mV for the DOX-loaded micelles, showing favorable stability. The DOX-loaded micelles could behave redox-sensitive drug release behavior in the solution containing 10 mM GSH. In vitro cytotoxicity showed that the bare micelles were biocompatible and DOX-loaded KSD micelles were more cytotoxic against tumor cells than normal cells.
