Isolation and Characterization of a Chitin-Degrading Strain Chitinibacter sp. GC72 and Identification of its Chitinase Degradation

Abstract:

A novel chitin-degrading aerobe, Chitinibacter sp. GC72, was isolated from a soil sample from Nanjing, and was proved to have high chitinolytic activity. The enzyme activity could reach to 2 835 U/g at 72 h of culture time, and one liter fermentation liquid contained 910 U chitinase. Its chitinase hydrolyzate, further investigated by TLC、UHPLC、Q-TOF-MS, was confirmed as GlcNAc. In the experiments of chitin hydrolyzation, the GlcNAc yield was 79.6% by using 3.44 U chitinase to hydrolyze chitin（200 mg） in 27 h.