Transcriptome Analysis of Torulopsis glabrata Under Hyperosmotic Stress

Abstract:

Gene expression profiles of Torulopsis glabrata at different osmolarity(860, 1 765, 2 603 mOs mol/kg and 3 324 mOs mol/kg) were investigated by microarray. Significantly upregulated genes were mainly involved in regulation of cell wall, cell membrane and pheromone-regulated membrane proteins. The genes in central metabolic pathway, such as glycolysis, Krebs cycle and oxidative phosphorylation pathway, were mainly upregulated, especially the genes associated with ATP or NADH formation. Futhermore, the genes associated with potential key compatible solutes, such as proline, arginine and glutamate synthesis pathway were upregulated, and urea carboxylase which involved in arginine catabolism was drasticly downregulated to only about 1/100.