Abstract:Postnatal 6 week ICR mice were exposed to 0.3 g/L caffeine solution for 21 days. After exposure, the influence of caffeine on intestinal villi morphology, expression of sweet receptors and taste signal transduction protein, release of insulin and GLP-1, glucose tolerance of mice were detected respectively by using the methods of stereomicroscope observation, western-blotting, ELIASA and blood glucose measurement technology. Compared with the control, the height of intestinal villi increased, the depth of crypt of intestinal villi decreased, thereby the ratio of villi and crypt of mice after long-term chronic caffeine exposure. Besides, long-term bitter taste exposure of mice increased the expression of TIR2 and TIR3, but decreased the expression of taste signal transduction molecule α-gustducin, however had no remarkable effect on the expression of SNAP 25. In addition, long-term caffeine exposure significantly increased the release sensitivity of insulin and GLP-1 of mice intestine, so notably increased the blood glucose tolerance of mice. The results indicated that caffeine exposure could alter the sweet perception pathway in intestine, and affect the blood glucose homeostasis of mice.
