Abstract:A total of 14 strains were screened that they initially isolated from homemade traditional fermented milk, which was sourced from Inner Mongolia, China. The results showed that all strains could remove cholesterol from culture medium of MRS-CHOL(P<0.05), and the percentage of Cholesterol-degrading rate ranged from 6.84% to 42.97%. Among them, strain N59-1 was selected after screening, which was based on their cholesterol removal abilities. The cholesterol removal rate was 42.97％. The strain N59-1 with the highest level of cholesterol removal from the media was identified using physiological and biochemical characteristics and 16S rDNA sequences, the homology of N59-1 with L. plantarum was 99.7%. As a result N59-1 was named L. plantarum. 30 days feeding test results showed that the organ growth and blood biochemical index of mice in each group had no significant difference compared with the control group(P>0.05).
