Abstract:To improve the specific growth rate and CO2 fixation rate of Spirulina platensis, the research was divided into four growth period: lag phase, the early of log phase, the end of log phase, the stable phase.The purpose was to optimize two main environmental factor which is light intensity and CO2 concentration. Experimental results showed that the growth of Spirulina platensis was conditioned in a 1 L conical flaske under 30 ℃ of temperature and 16/8 of light period: the optimum condition of lag period is light intensity 3 000 lux, without bubbling the CO2; the optimum condition of the early of log phaseis light intensity 4 000 Lux, with bubbling 15% concentration of CO2; the optimum condition of the end of log phase is light intensity 5 000 Lux, with bubbling 15% concentration of CO2; the light intensity the stable phase is lower than 3 000Lux, no need to bubble CO2. In continuous cultivation of Spirulina platensis regulate culture conditions of four period, the maximum biomass and carbon fixation rates were higher than the control group at 4.126 g/L and 58.231 mg/(L·h) respectively.