Effects of Saponification on the Contents of Carotenoids in Wolfberry Pomace

Abstract:

Ultrasonic-assisted Saponificationmethod was used to extract carotenoids from wolfberry pomace and the contents of carotenoids were detected. Based on single factor tests of different potassium hydroxide congcentration, temperature, time and ultrasonic power, the optimum extration process was evaluated by Box-Behnken central composite experimental design and response surface methodology analysis. The results showed that the optimal saponification processing parameters of carotenoids as followed: concentration of potassium hydroxide 1.8 mol/L, saponification temperature 40 ℃, saponification time 1 h, ultrasonic power 205 W. Under these compared with the predicted value 128.099 mg/hg, which indicated the degree of fitting was good and the saponification processing was accurate and reliable.