Cloning and Sequence Analysis of ACC I Oxidase Gene from Zizyphus jujube（Goutouzao）

Abstract:

In order to carry out researching of delaying Goutou jujube fruit ripening by regulation of ethylene synthesis with RNAi technology, a pair of specific primers were designed according to ACC I oxidase gene sequence of Zizyphus jujube(Dongzao) recorded in GenBank. Using test-tube plantlet total DNA from "Goutou jujube" as template, a fragment of ACC I oxidase gene including an ORF(1 294 bp) was amplified. The results showed that the fragment has four exons and three introns, turn in at 106~201, 430~541 and 877~1 002 bp base. Compared with nucleotide sequence and amino acid sequence homology of ACC I oxidase gene(EU216549.1) landed were 99.69%, which showed the successful cloning of the Goutou jujube ACC I oxidase gene.