Effect of Combination of Berberine and Ginsenosides Rb1 on Inflammatory Adipocytokines and Inflammatory Signaling Pathways in Adipocytes

Abstract:

The differentiated 3T3-L1 adipocytes were stimulated with/without tumor necrosis factor-α(TNF-α), then treated with berberine, ginsenosides Rb1, and both berberine and ginsenosides Rb1. The expression of TNF-α, interleukin-6(IL-6), resistin, adiponectin, and monocyte chemoattractant protein-1(MCP-1) were detected with real time quantitative PCR. The phosphorylation of IKK-beta and JNK were examined with western blotting. The results indicated that berberine can inhibit the expressions of inflammatory factors and inflammatory signaling transduction in adipocytes, but ginsenoside Rb1 doesn't produce obvious inhibitory effects, while rosiglitazone used as positive control drug. When combined with ginsenoside Rb1, the inhibitory effects to 3T3-L1 adipocytes of berberine are weakened, either with or without the stimulation of TNF-α.