Abstract:The research aimed to study the effect of Dendrobium huoshanense polysaccharides on the growth of human gastric cancer cell SGC-7901 and explore the correlation between the antitumor activity of polysaccharides and the expression of related genes. MTT assay of the growth of SGC-7901 cells treated with different fractions and concentrations of polysaccharides from D. huoshanense showed that total polysaccharides of D. huoshanense(DHP) and its two fractions (DHP-1 and DHP-2) could significantly inhibit SGC-7901 cells growth in a time- and dose- dependent manner. Light and laser scanning confocal microscope observation found that DHP-2 treatment caused SGC-7901 cells to become shrunk and apoptotic. qRT-PCR analysis displayed that DHP-2 not only obviously reduced the expression of gene c-myc, which were only account for 42.34% that of control, but also vastly increased the expression of gene p53, which was 1.04 fold higher than that of control. These results suggested that the inhibitory effect of D. huoshanense polysaccharides on human gastric cancer cell growth depends on its fraction, concentration and treatment time, which might be mediated by down-regulation of gene c-myc expression and up-regulation of gene p53 expression.
