Abstract:Analyzing the genetic relationships of the main Auricularia auricular cultispecies in Qinba mountainous area by ITS sequence so as to lay a theoretical foundation to genetics and breeding. Using CTAB method to extract genomic DNA from the bacterial strain, Auricularia auricular, and amplifying and sequencing PCR products; Auricularia auricula-judae 2 and Auricularia auricula-judae HF-8(GQ168492) were clustered in the same branch, Auricularia auricula-judae 913 and Auricularia auricula-judae YBS-7(GQ168503) were clustered in the same branch, but the genetic relationship between Auricularia auricula-judae Shennong A8 and the Auricularia auricula-judae 2, Auricularia auricula-judae 913 were relatively alienative. Auricularia auricula-judae 97-2, Auricularia auricula-judae NW482(EU520194), the Auricularia auricula-judae 268 and Auricularia auricula-judae NW491 (EU520160) were clustered in the same branch. However the genetic relationships among Auricularia auricula-judae Qindan 4, Auricularia auricula-judae 97-2, Auricularia auricula-judae 268 were relatively alienative. The genetic relationships among Auricularia auricula-judae Xinke, Auricularia auricula-judae 2, Auricularia auricula-judae 913 and Auricularia auricula-judae Shennong A8 were close. However, the relationships between Auricularia auricula-judae 268, Auricularia auricula-judae Qindan 4# and Auricularia auricula-judae 97-2 were quite far. Through the morphologic and ITS analysis of Auricularia auricular strains, two dominant strains, Auricularia auricula-judae Qindan 4# and Auricularia auricula-judae Shennong A8, have alienative relationship. Our findings established a solid foundation to genetics and breeding.
