Study of Comparison the Commonly Used Artificial Antigen Identification Methods

Abstract:

Research purpose of the paper was comparing the commonly identification methods of Artificial antigen, such as Native-PAGE electrophoresis, SDS-PAGE electrophoresis and UV spectro-photometry. In the paper, as the fungicide chlorothalonil was the based compound to preparate the artificial antigens. Native-PAGE electrophoresis, SDS-PAGE electrophoresis and UV spectro-photometry were used of qualitative analysising the artificial antigens, respectively. The results showed that the UV spectrophotometry identification method might cause the false positive, but it still could calculate the coupling ratio of the artificial antigen. If the false positive artificial antigen was used as immunizing antigen, the titer of antibody be low, while, the recognition ability with the carrier protein be strength, which is not suitable for establishing the immunoassay technology.