Optimization of Fermentation Conditions for Carotenoid Production by Rhodotorula glutinis Using Laser Tweezers Raman Spectroscopy Technology and Response Surface Methodology

Abstract:

Raman spectroscopy has been used widely in the field of biology in recent years due to its characteristics of fast, real-time and non-destructive analysis. In this paper, laser tweezers Raman spectroscopy(LTRS) was applied to detect the carotenoid content in Rhodotorula glutinis cells. Based on this, Plackett-Burman design was applied for evaluating effects of related factors on carotenoid production of Rhodotorula glutinis fermentation. Results showed that the factors including glucose, tryptone, pH and temperature presented the most major affecting on carotenoid yield. Then Box-Benhnken Design combined with response surface methodology was employed to optimize the most important effect factors of carotenoid yield. The optimal fermentation conditions for high carotenoid production consisted of 42.86 g/L glucose, 5 g/L tryptone, 5 g/L yeast extract, 1 g/L potassium dihydrogen phosphate, 0.5 g/L Magnesium sulfate, pH 7.0 with the temperature at 28.3℃. This optimization resulted in an increase of carotenoid yield with an improvment of 45% when compared with no optimizing.