Encapsulation and Release of Paclitaxel with Amphiphilic Random Polymer P（MMA-co-MAA） Micelles

Abstract:

The amphiphilic random polymer P(MMA-co-MAA) micelle was firstly applied to encapsulate paclitaxel with dialysis method. Critical micelle concentration(CMC) of amphiphilic random polymer P(MMA-co-MAA) was measured using the fluorescent probe method. The Zeta potential and the size of polymeric micelles were measured to compare loading micelle with blank micelle. The influences of the amounts of paclitaxel on encapsulation efficiency and loading content were investigated. Drug release was also studied in a phosphate buffer solution (pH 7.4). The result showed CMC of P(MMA-co-MAA) was 0.050 mg/mL as the monomer ratio of the hydrophobic monomer MMA to the hydrophilic monomer MAA was 7∶3. The encapsulation efficiency and loading content were 85.2% and 18.0%, respectively, as the content of paclitaxel added in polymeric micelle was 21%. Once loading paclitaxel, polymeric micelle became about twice as big as the blank micelle; and Zeta potential became more negative. The loading micelles release paclitaxel stably in 140 hours in phosphate buffer, which revealed that the paclitaxel loading micelles had an effective sustained-release effect.