Screening and Identify of a Strain Producing GABA by Enzymatic Conversion and Optimization of Fermentation Conditions

Abstract:

23 strains are isolated from five kinds of yoghurt and pickles. Six strains were obtained by screening comprised approach of TLC and HPLC .The high-yield GABA producing strain S9 was identified as Lactobacillus plantarum based on individual form, colony characteristics, physiological and biochemical characteristics and molecular biology features. Optimized fermentation medium is just as below: carbon source is sucrose(15 g/L), nitrogen source is compound nitrogen source(25 g/L), initial pH 6.2, 1 mmol/L Zn2+ and Mn2+, monosodium glutamate(10 g/L). Under the condition of the medium before, the best cell age is 20 h. The final GABA conversion is 3.9 g/L.