Separation and Identification the Nucleoside Components From Rape Bee Pollen

Abstract:

Rape bee pollen is crushing by grinder, then extraction with deionized water, take appropriate amount of the extract, through the macroporous resin column of AB-8 to crude separation, and then isolated by the MCI-GEL column and the ODS-B column , and at last I get three pure compounds. These three compounds are Being analyzed by 1H-NMR, 13C-NMR and 135DEPT-NMR, These three compounds were: guanosine, adenosine, deoxycytidine. These three kinds of nucleosides is the first time direct purification and structural identification from rape bee pollen.