Abstract:In order to explore the relationship between chlorophyll content and color changes of post-harvest broccoli in micro-vacuum conditions,a prediction model for chlorophyll content was established based on color parameter changes,freshly broccoli was stored in micro-vacuum conditions of pressure of 53.33 ~66.66、66.66 ~79.99、79.99 ~93.32 kPa and atmospheric pressure, temperature of(3±0.5) ℃,a predictive model of chlorophyll degradation was established through measuring the color parameter-a/b value,chlorophyll content changes and regression analysis. The results showed that:under 53.33~66.66、66.66~79.99、79.99~93.32 kPa and atmospheric pressure, kinetic models of-a/b value and chlorophyll content with respect to storage time were established according to the test data:C chl 1 =-0.008 5t+0.945 4,C chl 2 =-0.070 6t+0.756 4,C chl 3 =-0.071 4t+0.726 4, C chl 4 =-0.081 2t0.782 9 和-a1 /b1 =-0.014 3t+1.370 8,-a2 /b2 =-0.010 68t+1.456 8,-a3 /b3 =-0.127 1t+ 1.452 1,-a4 /b4 =-0.146t +1.519 3,they all met zero order kinetic model. In addition, prediction models for chlorophyll content based on-a/b value were further developed according to the linear relationship between chlorophyll content and-a/b value:C chl 1 =0.598 5(-a/b)-0.108 7(R2=0.997 6), C chl 2 =0.608 7(-a/b)-0.149 3(R2=0.9817),C chl 3 =0.559 3(-a/b)-0.083 7(R2=0.997 7),C chl 4 =0.5319(-a/b)-0.035 1(R2=0.986 9);they were feasible to use the color parameters changes to predict the chlorophyll content of broccoli after verification. Results of the study provided good support for the non-destructive testing of broccoli.