Identification of One Unknown Component and Detection Analysis of Free Amino Acids in Porphyra haitanensis

Abstract:

Free amino acids were separated by ion-exchange column chromatography from the crude extracts of Porphyra haitanensis. Amino acids were eluted with 3 mol/L NH3 ·3H2 O and the eluate was collected. Many protein amino acids and five unknown components were discovered in Porphyra haitanensis by thin layer chromatography,ion chromatography and PITC-HPLC respectively. One unknown derivative component(TZC) was obtained by preparation HPLC. TZC was white flocculent solid after vacuum freeze-drying. According to analysis of NMR,HR-MS,IR spectroscopy data,the molecular structure of TZC had been elucidated. TZC was ethanolamine after removed substituent component of PITC,its molecular formula was C2 H7 NO,molecular weight was 61.08.