Comparison of Proliferation from Rabbit Bone and Pig Bone Hydrolysates on Spleen Lymphocyte in Mouse

Abstract:

Fresh pig,fresh rabbit bone were enzymolyzed by trypsin,papain or Alcalase to obtain collagen peptides.The proliferation of hydrolysates on spleen lymphocyte in mouse was selected as an indicator.The best hydrolysis conditions were got bysingle factor experiment and orthogonal experiment.Results indicated that the optimal condition of pig bone were Alcalase,4 h(t),pH 9.5,6 000 U/g(E/S),45 ℃(T) and 6%(S). The proliferation rate of hydrolysates from pig bone on spleen lymphocyte was 83.41%. The optimal condition of rabbit bone were papain,4 h(t),pH 5.5,7 000 U/g(E/S),65 ℃(T) and 6%(S). The proliferation rate of hydrolysates from rabbit bone on spleen lymphocyte was 80.70%.