Abstract:Long-term effects of free and bound fructose on inflammatory factors and viscera histopathology in Sprague-Dawley rat were investigated. Male rats were randomized into four experimental groups and a control group. Each group was fed a diet containing 68% of total calories as different composition of carbohydrate for 11 weeks. At the end of the experiment,rats were sacrificed by anesthesia and were autopsied. Serum monocyte chemoattractant protein-1(MCP-1) and tumor necrosis factor-α(TNF-α) were detected by ELISA method. The pathological changes of liver and pancreas were directed with hematoxylin & eosin(H&E) staining. The results showed that the levels of serum and liver TNF-α and MCP-1 in fructose-fed rats were higher than the control.Moreover,the bound fructose(i.e. sucrose) showed higher serum MCP-1 than the free fructose(F/G)(P <0.05). Meanwhile,all of the three fructose containing diets induced various levels of hepatic steatosis,especially the SU and F/ST diet showed more severe fatty liver than F/G. The impairment of pancreas was unobvious,only the SU and F/ST groups showed slightly granular degeneration. It was suggested that long-term intake of high dose of fructose maybe induce inflammation and liver impairment,and bound fructose might produce more severe damage to health than the free fructose.
