Abstract:The aim of this manuscript was to evaluate antioxidant capacity and analyze the active substance of playing an important contribution to antioxidant capacity of Phellinus. Antioxidant activities and the contents of antioxidant substances of Phellinus were determined by various of antioxidant assay methods,and the correlative relationships between contents of different active components,between results from different antioxidant assay methods and between contents of different active components and results from different antioxidant assay were further investigated. The results showed that ws2 and ws7 had the highest contents of polysaccharide,flavonoid and phenol,and the correlative relationships between contents of different active substances had a large difference whether different genotypes or the same genotypes. Strains of ws2 and ws7 had the strongest scavenging capacity of hydroxyl radical,DPPH,superoxide anion radical,chelating ability and reducing power,and the results from different antioxidant assay methods had differently statistical correlation. The correlative relationship between content of total phenolic and results from different antioxidant assay methods was the strongest than those of polysaccharide and flavonoid,flavonoid’s taking second place,polysaccharide’s low correlation,which described that phenolic played a major contribution to the antioxidant capacity.