Optimization of Immobilization of Transglutaminase on Polypropylene Membrane

Abstract:

The factors of chemical immobilization of transglutaminase on polypropylene membrane were studied. The conditions of graft polymerization were optimized by using response surface methodology,and the optimal conditions of immobilization were determined by experiments. The results indicate that the optimum graft polymerization conditions as followings:distance of ultraviolet was 10.1 cm,consistency of methyl-methacrylate was 18.85%,and the time of ultraviolet was 21.4 minutes. The best grafting degree of the membrane was 34.62%. The conditions of immobilization was:consistency of hexamethylendiamine was 20%,at 50 ℃ for 90 min and consistency of glutaraldehyde was 2%,at 30 ℃ for 40 minutes,consistency of enzyme was 15 mg/mL,at 4 ℃ for 24 hours.