Abstract:In this manuscript,the effect of Staphylococcus aureus DNA hybridize special molecular beacon on the fluorescence intensity was determined to designed a probe to sensitive to Staphylococcus aureus DNA. The method using unique molecular beacon combined with ATMND dye which embed into absent site to hybridize complementary Staphylococcus aureus specific DNA sequence,resulting in stem-loop structure phase change and the ATMND dye is excited fluorescence. Different concentration of Staphylococcus aureus DNA hybrid with designed molecular beacon and observe the changes of fluorescence intensity. Finally,the standard strains DNA were used to verify the sensitivity to the probe. The probe has high sensitive to Staphylococcus aureus target DNA,along with high concentration of target DNA. Compared with other Salmonella,Escherichia coli,Enterobacter sakazakii,Listeria monocytogenes and Pseudomonas aeruginosa,the probe has more obvious fluorescence recovery ability. The fluorescence signal increased along with the increase of Staphylococcus aureus DNA concentration,the probe has a certain specificity in identification of Staphylococcus aureus DNA.
