Abstract:The purpose of this study was to extract and purify one lectin from the Pleurotus ferulae Lenzi,the basic physical and chemical properties of lectins were obtained,and build a solid foundation at the further PPL’s exploration in the immunomodulatory activity in theory. The highly purified lectin received through PBS extraction of fruit body mash,salting out with ammonium sulfate,freeze-drying,ion-exchange chromatography and Sepharose-4B affinity chromatography. Determinate the titer of hemagglutination by mice blood and the hemagglutinating effect as preliminary study on the physical and chemical properties of the lectin. The hemagglutination activities of PPL was destroyed after treatment with temperature higher than 70℃ or the pH lower than 2 and higher than 10. The qualities of the lectin are stable in the pH 4~9. when the Salinity increase,the activity decrease and D-galactose is the best inhibitors of letins,cations have different effects on the hemagglutinating activity of lectin.
