Breeding of Torula sp. B84512 Haploid and Analysis of Erythritol Production Characteristics

Abstract:

The Torula sp. B84512 was used for the industrial production of erythritol. To obtain a haploid genetics hybrid breeding of Torula sp. B84512,spore-producing cultivation of B84512 were done after cultured on Mcclary medium for 7 days at 30 ℃,and the sporulation rate could reach to 45%. Lysing the ascospore cell with snail enzyme for 150 min,10 haploid strains were screened. Furthermore,3 of them exhibit the highest ability to produce erythritol,and named as Torula sp. B84512-7,B84512-8 and B84512-9,respectively. With the PCR verification,Torula sp. B84512-7 and B84512-8 were verified as alpha type but Torula sp. B84512-9 was a type. The erythritol production of heterozygous Torula sp. B84512-79 reached at 97 g/L which was 56% of that of the parental strain. Therefore,Torula sp. B84512-7 and Torula sp. B84512-9 could be used for the further genetic manipulation.