Green and Nontoxic Enzyme-Linked Immunosorbent Assay for Aflatoxin B1 Detection

Abstract:

A environmental-friendly and nontoxic enzyme-linked immunosorbent assay(ELISA) for the detection of aftoxin B1(AFB1) in wheat flour sample was developed. In this method,the standard substance was substituted by the anti-idiotype antibody(anti-id antibody). The anti-id antibodies were prepared by immunizing the rabbits with the F(ab’)2 fragments that were obtained by digesting the monoclonal antibodies(11A9 and 1G3) against AFB1. Two kinds of anti-id antibodies was prepared and both could mimic the three-dimensional structural of AFB1 and caused specific inhibition to the 11A9 mAb. The correlation of the AFB1 and anti-id antibodies were analyzed by regression. The green ELISA based on anti-id antibody was applied in the analysis of spiked wheat flour sample. The recovery intra-and inter-assay was between 111.89% and 126.98%,respectively,while the CV less than 10%. The results indicated that the environmentalfriendly and nontoxic ELISA could be used for actual samples.