Abstract:With the developing of chromatographic separation,it is inevitable that cypermethrin isomers with high insecticidal activities would be prepared. In this work,HP-silica column and Sino-Chiral OD column were chosed as the stationary phase and examined using the ultraviolet detector to separation and isomer’s preparation of cypermethrin by the liquid performance spectrometer of Waters 484. Then,the configurations of cypermethrin isomers were identified according to the separation results by Sino-Chiral OD column and the reported studies. Finally,the inhibition effect of monoclonal antibodies to cypermethrin isomers was performed. The results demonstrated that only two of eight cypermethrin isomers,CP1(1R-cis-R) and CP2(1R-cis-S), were recognized by the antibodies,and CP1 was more sensitivity than CP2. Fifty percent inhibition concentration of CP2 with high insecticidal activities was 23.62 ng/mL. This results indicated that immunological technology for the determination of CP2 isomer could be established in the future.
