Analysis of Selenium Speciation in the Selenized Yeasts by RPLC-ICP-MS

Abstract:

The aim of this study is to detect the selenium speciation in the selenized yeasts. For this,to hydrolyze the proteins in the selenized yeasts through enzymatic digestion and to extract various amino acids composed the proteins in the yeats,the selenized amino acids were mensurated by reverse phase ion pair liquid chromatography and inductively coupled plasma and mass spectrometer(RPLC-ICP-MS),then the species of the selenized amino acids were confirmed. It was found that Selenomethionine was confirmed primary speciation of selenized amino acids in thesolutions of proteins hydrolyzed by enzyme through comparing the retention time of the amino acids with standard selenized compounds in the chromatography column. Conclusion:The method to extract the amino acids from proteins hydrolyzed by the enzyme and determination technology of RPLC-ICP-MS were suitable for analysis of selenium speciation in the selenized yeasts.