Abstract:In order to study the Ultrasound-assisted extraction technology of Sphallerocarpus gracilis stem leaves polyphenol and antioxidant activity. Response surface methodology(RSM) was used to establish the mathematical model of solvent extraction extraction to obtain the optimum conditions. The antioxidant activity of the extraction was evaluated by spectrophotometry on its ability of scavenging hydroxyl radical(·OH),1-diphenyl-2-picrylhydrazy(DPPH·) and total reducing power. Based on Box-Behnken design,the optimal process parameters were obtained as:ethanol concentration 75%,extraction temperature 70 ℃,ratio of solid to liquid(g/mL)35,ultrasonic time 30 min. Under the optimal condition,the average yield of polyphenol was 1.1448%.Results indicated that the scavenging effects of the extract on ·OH,DPPH· were significant,the extraction also had slightly total reducing power. And within 0.1 ~0.6 mg/mL concentration,the scavenging effects of Sphallerocarpus gracilis stem leaves polyphenol on·OH,DPPH· was higher than BHT. The Sphallerocarpus gracilis stem leaves polyphenol with good antioxidant activity has the potential to be further developed and utilized as a natural antioxidant.
