Comparative Study of Nucleic Acid Extraction Method of L.monocytogenes in the Different Kinds of Food Matrix

Abstract:

Compare the methods of extracting nucleic acid of Listeria monocytogenes in four food matrices. Taking L.monocytogenes as our research object,we picked and tested four widely applicable nucleic acid extraction methods and evaluated their performance by real-time PCR.Results:Tiangen centrifugal column method was very stable,with minimum variation caused by different food matrices. Promega precipitation and magnetic bead methods were more affected by food matrices than others,with a larger variation in final products;Chelex-100 method was quick and convenient,but was only suitable for pure liquid culture or colonies.