Identifing of Streptomyce HMJ-7 Producing Antibacterial Substance and Analysis of the Antibacterial Substance Stability

Abstract:

For obtaining new antibiotics and applying it to food preservation,A Streptomyce producing high antibacterial substance was screened from a sample obtained from Xuzhou kui river of Jiangsu. This strain was identified through it’s morphological,physiological and biochemical properties. 16S rDNA sequence analysis of HMJ-7 showed this strain was closely to Streptomyces griseoruber to 99%,So,it was identified as Griseorubro violaceus. The antibacterial experiment result showed that the antibacterial substance can obviously inhibit the growth of E. coli,Staphyloccocus aureus Rosenbach,Micrococcus luteus,Bacillus cereus and yeast. But can’t inhibit the growth of Aspergillus niger,Aspergillus flavus,Aspergillus oryzae and other fungi. The following experiment result indicated that the antibacterial substance produced by this strain was stable at differen temperature,pH,ultraviolet light and organic solvent condition.