Synthesis and Characterization of Antigen of Furazolidone

Abstract:

In order to prepare complete antigen against 3-amino-2-oxazolidinone(AOZ),which is one of main metabolites of furazolidone in animal,AOZ was synthesized using hydrazine and dimethyl carbonate. Then,3-(4-carboxy benzyl methylene)-amino-2-oxazolidinone(CPAOZ) was prepared as hapten based on AOZ. Three different methods including water-soluble carbodiimide method(EDC),condensation esterification(DCC) and mixed anhydride method(MA) method were used to prepare complete antigens by coupling CPAOZ to ovalbumin(OVA). All the products were characterized with UV spectrum. The coupling ratios of three artificial antigens were respectively 8、12、17. Under the optimized conditions,the prepared antigens were used to establish inhibition curves of AOZ and CPAOZ by enzyme-linked immunoassay(ELISA). Results showed that the lowest half inhibition concentration(IC50) of AOZ was 1.1 ng/mL sourced from coating antigen prepared using mixed anhydride method(MA) method.