Determination of Shikimic Acid in the Production of Microbial Fermentation

Abstract:

The method for determination of shikimic acid in the production of microbial fermentation was established. Using ultraviolet-visible spectrophotometry for determination of shikimic acid in the fermented liquid of shikimic acid producing bacteria. The detection wavelength was 245 nm,Linear equation of shikimic acid standard solution was A=3.084 4C-0.004 8(R2=0.9999). The recovery was 96.7% ~103.7% with average recovery of 100.04%,RSD was 0.32%. The method is simple,fast,stable and accurate,and suitable to determine the shikimic acid in the production of microbial fermentation.