Abstract:To explore the effect of Cynanchum auriculatum on melanin synthesis. The polysaccharides,C21steroidal glycosides,and phosphatide were extracted and separated from Cynanchum auriculatum. The effects of polysaccharides,C21steroidal glycosides,and phosphatide extracted from Cynanchum auriculatum on mushroom tyrosinase activity were detected respectively by measuring the oxidation rate of l-dopa. The results indicated that the soluble polysaccharides of Cynanchum auriculatum could inhibit the activity of mushroom tyrosinase obviously and concentration that resulted in 50% activity lost for diphenol oxidase activity was 31.8 μg/mL. The polysaccharides from Cynanchum auriculatum was a reversible competitive inhibitor for the oxidation of l-dopa. The phosphatide extracted from Cynanchum auriculatum had weak inhibitory effect on activity of mushroom tyrosinase while the C21steroidal glycosides had activation effect on the tyrosinase. The results suggest that polysaccharides of Cynanchum auriculatum might be used as a skin whitening agent or as a hyperpigmentation inhibitor. It also have the potential to be further developed into a safe and effective food browning inhibition agent.
