Studies on Anti-Tumor Activity in Vivo and Its Mechanism of A Polysaccharide Named IOP3a From Inonotus obliquus

Abstract:

The aim of this manuscript was to check the antitumor activity of IOP3a and to elucidate the anticancer mechanism in vivo with cancer nude mice transplanted jurkat tumor as model.. it was found that tumor weight of high-dose and middle-dose had significant differences(p<0.05),when compared with the control group. Inhibition rate of tumor was 69.28% and 57.62%,respectively,which had an excellent dose-response relationship. IOP3a could increase the spleen index,white blood cell and lymphocyte with the increase of dosage. IOP3a promoted generation of TNF-α and L-1β from macrophage of nude mouse obviously. The histopathology of tumors indicated that the tumor cells from the different IOP3a-treated groups had clear necrosis areas in tumor tissues.The anti-tumor mechanism of IOP3a in vivo was that IOP3a could improve the expression of tumor gene Bax and inhibit the the expression of tumor gene Bcl-2 with immuno-histochemistry.